. 2021 Jul 7;16(7):e0254251. doi: 10.1371/journal.pone.0254251

Table 1. Heterologous expression conditions and agonist (GABA) apparent affinity (EC₅₀).

Plasmid construct	Amount of	Day 1	Day 2	Day 3
(presence/absence 3’UTR)	cRNA (ng)	EC₅₀ (μM)	EC₅₀ (μM)	EC₅₀ (μM)
BtRDL (-3’UTR)	1	29.7 ± 1.1 (n = 7)	28.3 ± 1.1 (n = 6)	29.4 ± 1.0 (n = 6)
BtRDL (-3’UTR)	10	27.3 ± 1.1 (n = 8)	10.8 ± 1.0 (n = 6)	12.5 ± 1.0 (n = 8)
BtRDL (+3’UTR)	1	11.6 ± 1.1 (n = 7)	4.4 ± 0.7 (n = 9)	3.9 ± 0.6 (n = 8)
BtRDL (+3’UTR)	10	10.8 ± 1.2 (n-6)	2.0 ± 0.7 (n = 8)	1.8 ± 0.4 (n = 9)

Data are means ± SEM.

Table 1. Heterologous expression conditions and agonist (GABA) apparent affinity (EC50).