Fig 1. Nerve slices at 10x magnification demonstrating number of micrographs and stereology sample.

(A) Representative naïve nerve (osmium-paraffin protocol). Grey boxes represent individual micrographs, while the clear square in the middle represents the stereologic sampled area of 25x25 μm box (625 μm²) used for naïve samples. Axons touching the top and right borders of the box were included in the measurements while those touching the bottom and left borders were not. (B) Representative regenerating nerve (osmium-paraffin protocol), demonstrating stereologic sampling with larger 40x40 μm box (1600 μm²), given decreased axon density.