Fig 4. Bland-Altman plots of ADS and manual axon counts.

(A) In naïve nerves prepared with the toluidine-resin protocol, the limits of agreement between ADS and manual counts in the 625 μm² sample area in each micrograph ranged from -5 to 5. (B) In naïve nerves prepared with the osmium-paraffin protocol, the limits of agreement between ADS and manual counts in the 625 μm² sample area ranged from -6 to 6. (C) In regenerating nerves prepared with the osmium-paraffin protocol, the limits of agreement between ADS and manual counts in the 1600 μm² sample area ranged from -2 to 12.