Fig. 2.

2D in vitro model implies the cytotoxicity and induction of neuronal differentiation DYRK1A inhibition effect of EGCG. a EGCG reduced the cell viability of SY5Y in a dosage-dependent manner. b Western blotting showed decrease of DYRK1A protein expression following the treatment with EGCG in a dosage-dependent manner. c Western blotting showed decrease of DYRK1A protein expression after SH-SY5Y cells were treated with known DYRK1A inhibitors. d CRISPR-Cas9 knock-out of DYRK1A using four-designed single-guided RNA (sgRNA) significantly decreased the protein level of DYRK1A