Fig. 4.

EGCG treatment reduced the viability of SH-SY5Y 3D Matrigel sandwich in vitro model. a Whole culture fixation and immunofluorescence imaging showing the culture had a thickness of 200 µm with Beta-tubulin III-positive stained neurite-like structures growing from the culture (red fluorescence). b Representative microscopy images of SH-SY5Y cultured in Matrigel sandwich suggested that EGCG impaired DYRK1A expression (red fluorescence) in Matrigel 3D-cultured neuroblastoma. c Viability quantification showed significant difference between the control group and the group treated by 5 µM EGCG (n = 4). The experiments were repeated three times. Based on Students’ t test, **p < 0.01. (Color figure online)