Figure 1.

NONO is essential for DNA repair and radioresistance of tumor cells. A. NONO was knocked out in HeLa, MDA-MB-231 and U2OS cells. B. NONO depletion sensitized tumor cells to irradiation. C. NONO-KO cells-derived xenograft was more sensitive to irradiation than that from wildtype cells. Ten days after MC38 cell injection, mice were treated with radiotherapy (RT). D. NONO knockout inhibited NHEJ in in vitro NHEJ assay. NP, nuclear protein extracted from U2OS cells. E, F. NONO was overexpressed in breast cancer (BC) tissues, and high level of NONO was correlated with poor prognosis of breast cancer patients in TCGA data. G. NONO was upregulated in colorectal cancer (CRC). The mRNA level of NONO was analyzed in 49 paired CRC and normal (N) tissues using qPCR. H. NONO was highly expressed in radioresistant CRC tissues. The protein level of NONO was examined in CRC tissues with IHC. **, P < 0.01; ***, P < 0.001.