Figure 2.

NONO interacts with EGFR. (A) CoIP assay with myc-EGFR and Flag-NONO transfected HEK293T cells. (B, C) CoIP of endogenous NONO or EGFR protein in U2OS cells. (D) The association of NONO and EGFR was verified with Duolink PLA assay. Antibodies, anti-NONO and anti-EGFR antibodies. (E) NONO interacted with the intracellular domain of EGFR. (F) EGFR bound to RRM1 domain of NONO. For (A, E, F), 36 hours after transfection with indicated plasmid, HEK293T cells were collected and subjected to CoIP assay.