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. 2021 Jun 15;11(6):2838–2852.

Figure 4.

Figure 4

NONO is not phosphorylated by EGFR. (A) anti-phospho-tyrosine antibody (4G10) non-specifically bound to NONO. (B, C) EGF treatment (B) or irradiation (C) had no impact on the phosphorylation of NONO. LE: Long exposure. SE: Short exposure. (D) all of 5-tyrosine sites mutation had no impact on the phosphorylation of NONO. For (A-D), HEK293T cells were transfected with myc-EGFR and Flag-NONO plasmid for 36 hours before treated with IR (10 Gy) or EGF (10 ng/ml) for indicated times. For (B-D), the phosphorylated NONO was analyzed using Phos-tag-SDS-PAGE.