Table 1.
Primer sequences used for stem-loop RT-qPCR
| Primer | Sequence (5’-3’) | T ann (°C) |
|---|---|---|
| RT-Stem-loop-Rv | TGGTGCAGGGTCCGAGGTATT | - |
| RT-hsa-miR-19b-3p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACTCAGTT | - |
| RT-hsa-miR-19b-3p Fw | GGCGGTGTGCAAATCCATGC | 65 |
| RT-hsa-miR-320a-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCCGG | - |
| RT-hsa-miR-320a-5p Fw | GGCGGGCCAACACAACCCGGA | 65 |
| RT-hsa-miR-101-3p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACTTCAGT | - |
| RT-hsa-miR-101-3p Fw | CGCGCGTACAGTACTGTGATA | 62 |
| RT-hsa-miR-3613-3p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCGGT | - |
| RT-hsa-miR-3613-3p Fw | CCGGCCACAAAAAAAAAAGCCCA | 65 |
| RT-hsa-miR-1207-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCCCCTC | - |
| RT-hsa-miR-1207-5p Fw | GGCGGTGGCAGGGAGGCTGG | 65 |
| RT-hsa-miR-5095-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCGGT | - |
| RT-hsa-miR-5095 Fw | GGCGCTTACAGGCGTGAACC | 65 |
| RT-hsa-miR-2277-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGACTGG | - |
| RT-hsa-miR-2277-5p Fw | GGCGGAGCGCGGGCTGAGCGCTG | 65 |
| RT-hsa-miR-4497-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGCCCAG | - |
| RT-hsa-miR-4497 Fw | CGCGCTCCGGGACGG | 65 |
| RT-hsa-miR-320e-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCCTTCT | - |
| RT-hsa-miR-320e Fw | GCGCGGAAAGCTGGGTTG | 65 |
| RT-hsa-miR-4532-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCCGG | - |
| RT-hsa-miR-4532 Fw | GAGACCCCGGGGAGC | 65 |
| RT-hsa-miR-4668-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGACAAA | - |
| RT-hsa-miR-4668-5p Fw | GGCGGAGGGAAAAAAAAAAGGA | 65 |
| RT-hsa-miR-30b-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGCTGAG | - |
| RT-hsa-miR-30b-5p Fw | CCGCGCTGTAAACATCCTACAC | 70 |
| RT-hsa-miR-221-3p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGAAACC | - |
| RT-hsa-miR-221-3p Fw | GGCGGAGCTACATTGTCTGCTG | 65 |
| RT-hsa-miR-195-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACGCCAAT | - |
| RT-hsa-miR-195-5p Fw | GGGGGGTAGCAGCACAGAAAT | 65 |
| RT-hsa-miR-21-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACTCAACA | - |
| RT-hsa-miR-21-5p Fw | CGGGGGGTAGCTTATCAGACTG | 65 |
| RT-hsa-miR-16-5p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCGCCAA | - |
| RT-hsa-miR-16-5p Fw | GGCCCGTAGCAGCACGTAAATA | 65 |
| RT-hsa-miR-103a-3p-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACTCATAG | - |
| RT-hsa-miR-103a-3p Fw | GGGGAGCAGCATTGTACAGGG | 67 |
| RT-miR-Cel39-STEM | GTCTCCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGGAGACCAAGCT | - |
| RT-miR-Cel39 Fw | CGGGGTCACCGGGTGTAAATC | 65 |