Figure 4.

Inhibitory effect of SMI on ASPH enzymatic activity. (A) Scheme of in vitro metastasis assay. (B, C) In vitro metastasis modulated by WT- vs. mutant-ASPH: (B) Transendothelial migration (intravasation/extravasation); (C) Invasion through basement membrane and subsequent mammosphere formation. (D, E) ¹⁴CO₂/α-Ketoglutarate-Dependent Capture Assay. (F) Quantification of ASPH enzymatic activity in response to SMI. (G) Inhibitory effect of SMI (at high dose only) on cell viability. *P < 0.05; **P < 0.01; ***P < 0.001.