Figure 1.

Niclosamide inhibits cell proliferation and induces apoptosis in CRPC cells. (A) PC-3 and 22Rv1 cells were treated with the indicated concentrations of niclosamide for 48 hr and cell viability was measured using the WST assay. (B) Colony formation assays were performed in PC-3 and 22Rv1 cells incubated with or without 2.5 µM niclosamide. The colonies were photographed and the number of colonies was counted. (C) PC-3 and 22Rv1 cells were incubated with 2.5 μM niclosamide for 24 and 48 hr. Apoptosis was determined by analyzing FITC Annexin V-PI staining. All data are presented as the mean 48 hr. Apoptosis was determined by analyzing: **P < 0.01, ***P < 0.001, two-tailed Student’s t test or one-way ANOVA test.