Figure 2.

Structure of the genomes of OV-BiTE. A. vvDD (double-deleted VVs). The lacZ gene is inserted into its VGF site. vTK-L/R, the left and right segments of vaccinia TK gene; Pse/I, synthetic early/late promoter; F17R, late promoter. B. ICO15K (ICOVIR-15K). L/RITR, left/right inverted terminal repeats; SA, splicing acceptor; K, kozac sequence; C. EnAd (enadenotucirev). The Ad11p sequence between the base pair 6081 and 9322 in the E2B region is often replaced by the Ad3 sequence. In addition, the E3 region of EnAd is almost completely deleted, and the E4orf4 region of the virus has a 25 bp deletion. The BiTE transgenes were inserted in the downstream of a CMV promoter or splice acceptor site (P) followed by a polyadenylation sequence (pA). D. MV (measles virus). The transcription unit downstream of the leader sequence (ld) encodes enhanced green fluorescent protein (eGFP). F, H, L, M, N, and P respectively represent the coding genes for structural fusion protein, hemagglutinin protein, large protein (polymerase), matrix protein, nucleoprotein, P protein. BiTE was inserted into an ATU downstream of the H open reading frame. the Kozak sequence is located before the BiTE. To comply with the six-rule, other nucleotides are exemplarily included downstream of the coding region. Two restriction sites can be inserted into the corresponding ATU on both sides of the insertion box.