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. 2021 Jul 7;12:4170. doi: 10.1038/s41467-021-24407-y

Fig. 5. M1BP and CP190 both activate Motif 1-containing gene promoters.

Fig. 5

a Schematic diagram of plasmids containing wild-type and Motif 1 mutants of ribosomal protein (RP) gene promoter driving luciferase reporters. b Schematic diagram showing RP gene luciferase reporter assay. RpIII128 promoter lacks Motif 1 and serves as a transfection control. c Firefly/Renilla luciferase ratio of relative light unit measurements. Experiments are performed in indicated knockdown conditions. Data represented as mean ± SD from n = 3 biological replicates, and P values were analyzed by two-tailed unpaired t-test. WT RpLP1 Mock vs M1BPRNAi; Mut Motif 1 RpLP1 Mock vs M1BPRNAi; WT RpLP30: Mock vs M1BPRNAi; WT RpLP1 Mock vs Cp190RNAi; and WT RpLP30 Mock vs Cp190RNAi all P = 0.0001, Mut Motif 1 RpLP30 Mock vs M1BPRNAi P = 0.0017, Mut Motif 1 RpLP1 Mock vs Cp190RNAi P = 0.73, Mut Motif 1 RpLP30 Mock vs Cp190RNAi P = 0.89, WT RpLP1 Mock vs mod(mdg4)RNAi P = 0.49, Mut Motif 1 RpLP1 Mock vs mod(mdg4)RNAi P = 0.16, WT RpLP30 Mock vs mod(mdg4)RNAi P = 0.28, Mut Motif 1 RpLP30 Mock vs mod(mdg4)RNAi P = 0.98.