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. 2021 Jul 7;11:14001. doi: 10.1038/s41598-021-93430-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The mitochondrial function test of the mitochondrial damage model induced by d-Gal after UL16 transfection. (A) It was verified that UL16 had been successfully transfected into the HUVEC cells induced by d-Gal. (B, C) The intracellular ROS level was detected in d-Gal group and UL16 group. (bar.50 μm, *p < 0.05, **p < 0.01). (D) UL16 was transfected into HUVEC cells induced by d-Gal, and then the content of ATP was measured in UL16 group, d-Gal group, Noraml group and Empty group. (E, F) The mitochondrial membrane potential of cells in d-Gal group and UL16 group was measured. electron microscope. (bar.20 μm, *p < 0.05, **p < 0.01).