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. 2021 Jul 7;12:4195. doi: 10.1038/s41467-021-24326-y

Fig. 1. The binding affinity of RBD to both hACE2 and dACE2, and the infection efficacies of pseudovirus and authentic virus in hACE2- and dACE2-expressing BHK21 stable cells.

Fig. 1

a Schematic of SARS-CoV-2 S (first panel) and dACE2 (second panel) linear compositions colored per domain. b SPR-binding curves for immobilized hACE2 with the soluble RBD. c SPR-binding curves for immobilized dACE2 with the RBD. The black dashed lines represent the actual data, while the red solid lines represent the fitted results (b, c). d Infection of dACE2-expressing BHK21 cells with SARS-CoV-2 S protein-bearing pseudovirus. e Infection of hACE2-expressing BHK21 cells with SARS-CoV-2 S protein-bearing pseudovirus. ****p < 0.0001; ns means no significant differences; two-tailed Student’s t-test. The experiment was performed with quintuplicate cell samples (d, e). f Infection of dACE2/hACE2-expressing HeLa cells with SARS-CoV-2 authentic virus. The experiment was performed with duplicate cell samples. Data are presented as mean values ± SD (d–f).