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. 2021 Jul 7;12:4195. doi: 10.1038/s41467-021-24326-y

Fig. 4. The binding ability of RBD mutants to both soluble and transmembrane dACE2 and hACE2.

Fig. 4

a–c SPR sensorgram for RBD interface residue mutants binding to dACE2. e–g RBD interface residue mutants binding to hACE2. The black dashed lines represent the actual data, while the red solid lines represent the fitted results. The kon, koff, and KD values for each mutant are indicated. d, h Flow cytometry assay for RBD interface residue mutants binding to dACE2 (d) and hACE2 (h). The Y-axis represents the percentage of the APC+eGFP+ cells in the eGFP+ cells. Data are presented as mean values ± SD of triplicate cell samples. ****p < 0.0001 vs wt; two-tailed Student’s t-test.