Fig. 7.

Cyp2e1 in the granular cell region of the ipsilateral hippocampus. Astrocytes in the dentate gyrus region of the hippocampus were frequently found to stain positively at a moderate intensity in the soma (arrowheads) and sometimes more intensely with immunoreactivity throughout the branched processes (arrows). Hoechst 33,342 staining of nuclei is shown in the blue channel and Cyp2e1 immunoreactivity in the red channel in these example sections from mice with no insult or treatment (a), mTBI + smoke exposure without treatment (b) or mTBI + smoke exposure with the tBHQ treatment (c). The region is from the right hippocampus closest to the closed head impact centered at − 2.9 mm from bregma