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Safranin-O staining and immunohistochemistry of human neocartilage derived from donors 2 and 3. (a) Safranin-O staining, (b) type II collagen, and (c) type I collagen of neocartilage constructs derived from P5 and P9 donor 2 hACs, and from P3, P7, and P11 donor 3 hACs. The effects of no treatment (Ctrl), and aggregate rejuvenation followed by TCL treatment (Agg+TCL) on human neocartilage are shown.
