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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Prog Retin Eye Res. 2021 Jan 8;83:100941. doi: 10.1016/j.preteyeres.2021.100941

Figure. 9. UCP2 is protective against H2O2 and NO in PC12 cells.

Figure. 9.

A, RT-PCR amplification of UCP2 from a plasmid positive control (hUCP2), PC12 cells transfected with GFPalone (Con), and PC12 cells transfected with GFP plus hUCP2 (GFP_hUCP2), confirming expression only in the transfected cells. B–D, Images of cells transfected with GFP alone before (B) and after (C) treatment with H2O2 or transfected with both GFP and hUCP2 (D). All fluorescent spots represent cells, with the larger spots represent multicellular aggregates. Many more cells survive after hUCP2 transfection. E, Summary of three independent experiments, in each of which six separate wells were counted. Cell death is presented as the mean ± SE. After treatment with either H2O2 or NO, there is significantly less (P <0.05) cell death in cultures transfected with hUCP2. (Adapted from ref 11)