Figure 6.

Cd exposure increased the expression of p-JNK and BACE-1. (A) Western blot images against p-JNK and BACE-1 in the cortical and hippocampal regions of the brain and their respective histograms. β-Actin was used as a loading control. The histograms represented as the means ± SEM (n = 7 mice/group) for three repeated independent and reproducible experiments. Significance = ***p < 0.001; ^###p < 0.001 Student’s unpaired t-test. See Supplementary Figure 3 for uncropped and original blots. (B) Representative immunofluorescence images of p-JNK (green: FITC and blue: DAPI) in the cortical and hippocampal regions of the mouse brain. The histograms represented as the means ± SEM for n = 5 mice per group, and the number of independent experiments = 3. Magnification: 10×. Scale bar = 50 μm. Significance = ***p < 0.001; ^###p < 0.001. (C) The double immunofluorescence and co-localization of p-JNK (green: FITC and blue: DAPI) and BACE-1 (red: TRITC and blue: DAPI) in the hippocampal neuronal HT22 cells exposed to Cd (10 μM) and SP600125 (20 μM). The data are indicated as the ±SEM for n = 5 images per group, and the number of independent experiments = 3.