FIGURE 6.

Effects of mirabegron on KCl-precontracted human prostate tissues, and on EFS- and noradrenaline-induced contractions in the presence of L-748,377. In (A), prostate tissues were precontracted with 80 mM KCl. After a stable precontraction was attained, mirabegron was added in cumulative concentrations (1–10 µM). Shown are data from n = 5 patients together with means, which are expressed as percentage decrease from the KCl-induced precontraction (#p < 0.05 vs. precontraction before mirabegron by Dunn’s test). Note that KCl-induced contractions were set to 100% and all values before mirabegron reflect 0% relaxation (dotted line). In (B) and (C), contractions of prostate tissues from the periurethral zone were induced by EFS (B) or noradrenaline (C) in an organ bath, 50 min after adddition of L-748,377 (1 µM) to all tissues and 30 min after addition of mirabegron (10 µM) or an equivalent amount of DMSO (controls), which was used as solvent for mirabegron. Shown are data from n = 5 patients in each diagram, which are means ± SD in frequency response curves (#p < 0.05 for control vs. mirabegron by two-way ANOVA, and p values for whole groups in inserts from 1-way ANOVA).