FIGURE 8.

Effects of mirabegron on proliferation, viability and cAMP formation in human prostate stromal cells. In (A), proliferation was assessed by EdU assays (red, proliferating cells; blue, non-proliferating cells), following incubation of WPMY-1 cells with DMSO (controls) or mirabegron in indicated concentrations for 24 h. Proliferation is expressed as percentage of cells showing proliferation, referred to the number of all cells in a microscopic field. In (B), viability was assessed by CCK-8 assays, following incubation of WPMY-1 cells with DMSO (controls) or mirabegron in indicated concentrations for 48 h. Viability is expressed as extinction (OD), which is the final readout of CCK-8 assays. In (C), cAMP in lysates was assessed by ELISA following incubation of cultured stromal cells with 1 µM or 10 µM mirabegron for 30 min, and in corresponding controls. In (A) and (B), all single values together with means and p values (Dunnett’s test) from five independent experiments for each setting are shown, together with pictures from represenative experiments from EdU assay. In (C), all single values with means from four independent experiments are shown.