Table 2.
Target genes | Primer | Primary sequence (5′→3′) | Accession no. |
---|---|---|---|
Nrf2 | Forward | TGTGTGTGATTCAACCCGACT | NM_205117.1 |
Reverse | TTAATGGAAGCCGCACCACT | ||
HO-1 | Forward | TTGGCAAGAAGCATCCAGA | NM_205344.1 |
Reverse | TCCATCTCAAGGGCATTCA | ||
SOD1 | Forward | TTGTCTGATGGAGA TCATGGCTTC | NM_205064.1 |
Reverse | TGCTTGCCTTCAGGATTAAAGTGAG | ||
SOD2 | Forward | CAGATAGCAGCCTGTGCAAATCA | NM_204211.1 |
Reverse | GCATGTTCCCATACATCGATTCC | ||
GPx1 | Forward | GACCAACCCGCAGTACATCA | NM_001277853.1 |
Reverse | GAGGTGCGGGCTTTCCTTTA | ||
GPx3 | Forward | CCTGCAGTACCTCGAACTGA | NM_001163232 |
Reverse | CTTCAGTGCAGGGAGGATCT | ||
CAT1 | Forward | ACCAAGTACTGCAAGGCGAAAGT | XM_015277937.2 |
Reverse | ACCCAGATTCTCCAGCAACAGTG | ||
GSTT1 | Forward | GACGGAGACTTCACCCTAGCAGA | NM_205365.1 |
Reverse | TGATGGGTACCAGTGGTCAGGA | ||
GSTO1 | Forward | CATGATGTGGCCCTGGTTTG | NM_001277375.1 |
Reverse | CAGTGCTGGAGCTTTGGAGTATGA | ||
GSTA3 | Forward | TTGGATAAGGCCGCAAACAGATA | NM_001001777.1 |
Reverse | TTTCCAGTAAATGCACGTCTGCTC | ||
IκBα | Forward | GGCAGATGTGAACAAGGTGA | NM_001001472.2 |
Reverse | TATCTGCAGGTCAGCTGTGG | ||
NF-κB p65 | Forward | GTGTGAAGAAACGGGAACTG | NM_205129 |
Reverse | GGCACGGTTGTCATAGATGG | ||
TNF-α | Forward | GCCTATGCCAACAAGTACACCT | NM_204267.1 |
Reverse | GCCAAGTCAACGCTCCTG | ||
IFN-γ | Forward | CTTCCTGATGGCGTGAAGA | NM_205149.1 |
Reverse | GAGGATCCACCAGCTTCTGT | ||
IL-1β | Forward | CGCCGCTACCAGAGGGACTT | NM_204524.1 |
Reverse | CCGGACCCAGTTGACCCCAT | ||
IL-2 | Forward | ATCTTTGGCTGTATTTCGGTAG | NM_204153.1 |
Reverse | TCCTGGGTCTCAGTTGGTG | ||
IL-4 | Forward | CTCCTCACTGCCCACCCT | NM_001007079.1 |
Reverse | CATCTTGACGCAGGAAACCT | ||
IL-6 | Forward | GATCCGGCAGATGGTGATAA | NM_204628.1 |
Reverse | AGGATGAGGTGCATGGTGAT | ||
IL-10 | Forward | GCTCTCCTTCCACCGAAACC | NM_001004414.2 |
Reverse | GGAGCAAAGCCATCAAGCAG | ||
β-actin | Forward | TCAGGGTGTGATGGTTGGTATG | NM_205518.1 |
Reverse | TGTTCAATGGGGTACTTCAGGG |