Figure 2. Identification of the E375G polymorphism (Glu³⁷⁵Gly variant) in CAMKK1, detected by BseRI restriction enzyme.

Top: schematic presentation of CAMKK1 domain structure. The conserved CaM-kinase domain is shown in gray (catalytic domain), white (autoinhibitory domain) and black (CaM-binding domains). The little triangles show the amino acid position of E375G polymorphism (CTC→CCC) in the kinase domain of CAMKK1. In (a) it is shown that the T allele generates a restriction recognition site (RRS) for BseRI. Its cleavage creates two fragments of 114 and 212 bp. In (b) it is shown that the C allele does not generate an RRS for BseRI, thus it can not cleave. The result is a fragment of 326 bp. Bottom: Electrophoresis on 3% agarose gel of BseRI restriction products: Lanes 1, 4 and 7, TT genotype (212 and 114 bp); s 2, 3 and 6, TC genotype (326, 212 and 114 bp); lane 5, CC genotype (326 bp); lane M, DNA ladder.