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. 2021 May 23;100(8):101276. doi: 10.1016/j.psj.2021.101276

Table 8.

Fatty acids composition (% of total fatty acid methyl esters) of egg yolk from laying hens fed diets containing increasing levels of soluble flaxseed oil for 2 wk.1

Fatty acids Soluble flaxseed oil concentration in diets, %
 SEM P-value
0 0.2 0.4 0.6 0.8 ANOVA Linear Quadratic
Palmitic acid (C16:0) 26.75ab 26.49abc 26.88a 25.44c 25.68bc 0.37 0.034 0.012 0.558
Heptadecanoic acid (C17:0) 0.14c 0.16bc 0.16bc 0.19a 0.17ab 0.01 0.004 0.001 0.111
Oleic acid (C18:1n-9) 41.81 39.75 38.86 39.58 41.03 1.09 0.341 0.617 0.045
Linoleic acid (C18:2n-6) 14.76b 16.84a 15.65ab 17.01a 15.85ab 0.46 0.012 0.118 0.031
γ-linolenic acid (C18:3n-6) 0.11 0.13 0.11 0.12 0.11 0.01 0.120 0.694 0.186
α-linolenic acid (C18:3n-3) 0.25b 0.38a 0.39a 0.18b 0.19b 0.02 <0.001 <0.001 <0.001
Dihomo-γ-linolenic acid (C20:3n-6) 0.18 0.17 0.19 0.17 0.16 0.01 0.747 0.513 0.400
Eicosatrienoic acid (C20:3n-3) 0.01b 0.02b 0.02a 0.03a 0.03a 0.00 <0.001 <0.001 0.264
Arachidonic acid (C20:4n-6) 0.01abc 0.01a 0.01ab 0.01bc 0.01c 0.00 0.009 0.007 0.019
Eicosapentaenoic acid (C20:5n-3) 0.01 0.01 0.02 0.01 0.01 0.00 0.659 0.630 0.166
Docosahexaenoic acid (C22:6n-3) 0.66e 1.02d 1.28c 1.64a 1.45b 0.04 <0.001 <0.001 <0.001
Nervonic acid (C24:1n-9) 0.25a 0.13b 0.18b 0.19ab 0.05c 0.02 <0.001 0.000 0.410
Others2 15.06 14.88 15.97 15.43 15.14 0.62 0.753 0.717 0.430
Total SFA3 35.87 35.63 36.82 34.87 34.81 0.68 0.239 0.192 0.279
Total MUFA4 48.00 45.63 45.08 45.77 47.11 0.93 0.188 0.582 0.020
Total PUFA5 16.13c 18.74ab 17.91ab 19.36a 17.69ab 0.49 0.001 0.029 0.003
Total n-96 44.72 42.61 42.25 43.06 44.24 0.91 0.272 0.860 0.031
Total n-67 15.05b 17.15a 15.96ab 17.31a 16.13ab 0.47 0.013 0.130 0.030
Total n-38 0.93d 1.43c 1.74ab 1.86a 1.69b 0.05 <0.001 <0.001 <0.001
n-3:n-6 ratio9 0.06c 0.08b 0.11a 0.11a 0.11a 0.00 <0.001 <0.001 <0.001

Abbreviation: ANOVA, analysis of variance.

a,b,c

Mean value without a common superscript within the same row differ (P < 0.05).

1

Data were reported as % of total fatty acid methyl esters.

2

Others were calculated as C15:0 + C16:1 + C17:1 + C18:0 + C20:1n-9 + C20:2 + C22:1n-9.

3

SFA, saturated fatty acids; SFA level were calculated as C15:0 + C16:0 + C17:0 + C18:0.

4

MUFA, monounsaturated fatty acids; MUFA levels were calculated as C16:1 + C17:1 + C18:1n-9 + C20:1n-9 + C22:1n-9 + C24:1n-9.

5

PUFA, polyunsaturated fatty acids; PUFA levels were calculated as C18:2n-6 + C18:3n-6 + C18:3n-3 + C20:2 + C20:3n-6 + C20:3n-3 + C20:4n-6 + C20:5n-3 + C22:6n-3.

6

Total n-9 was calculated as C18:1n-9 + C20:1n-9 + C22:1n-9 + C24:1n-9.

7

Total n-6 was calculated as C18:2n-6 + C18:3n-6 + C20:3n-6 + C20:4n-6.

8

Total n-3 was calculated as C18:3n-3 + C20:3n-3 + C20:5n-3 + C22:6n-3.

9

Ratio of n-3:n-6 was calculated as Total n-3:Total n-6.