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. 2020 Apr 21;11(3):340–350. doi: 10.1016/j.jpha.2020.04.004

Fig. 1.

Fig. 1

CPT1C knockdown induces cellular senescence in PANC-1, MDA-MB-231, HCT-116 and A549 cells. (A) BrdU incorporation activity during DNA synthesis. Data are represented as mean ± SEM, n = 5/group (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001 vs. the siControl group). (B) Cell colony formation ability by staining with crystal violet after cultured at indicated dilutions. (C) Senescence associated β-gal activity assay. (D) qPCR analysis of senescence-associated secretory phenotype (SASP) mRNAs. Data are represented as mean ± SEM, n = 4/group (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001 vs. the siControl group). BrdU: bromodeoxyuridine; CPT1C: carnitine palmitoyltransferase 1C; SASP: senescence-associated secretory phenotype; IL: interleukin; TNFα: tumor necrosis factor alpha; TGFB1: transforming growth factor beta; MMP: matrix metallopeptidase.