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. 2021 Jun 24;9:692168. doi: 10.3389/fchem.2021.692168

FIGURE 5.

FIGURE 5

DSC measurements of 3CLpro variants. (A) DSC thermal scans of 3CLpro WT, and its His41 and Cys145 variants acquired at pH 7.0 and 20% (v/v) DMSO. The sample was heated at a rate of 1.0°C/min, and the scans of the variants were acquired in the absence (solid line) or presence (dotted line) of 50 μM peptide substrate. The DSC scan of WT enzyme was not acquired in the presence of peptide substrate, as the protease is catalytically active. (B) Bar plot of T m calculated at the apex of the melting peak in the absence (solid bars) and presence (checkered bars) of 50 μM peptide substrate. Colors are as in panel A. (C) Bar plot of ΔH cal calculated from the area under the thermographic peak in the absence (solid bars) and presence (checkered bars) of 50 μM peptide substrate. Colors are as in panel A. Data presented in (B) and (C) are shown as the mean + SD, n = 3.