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. 2021 Jun 24;14:681868. doi: 10.3389/fnmol.2021.681868

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Copyright © 2021 Molnar-Kasza, Hinteregger, Neddens, Rabl, Flunkert and Hutter-Paier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histological analysis of the musculus gastrocnemius of 24–30 weeks old SOD1-G93A/low mice. The musculus gastrocnemius of 24, 27, and 30 weeks old male SOD1-G93A/low mice was evaluated for region size (A), numerical density of acetylcholine receptors at the postsynaptic surface of neuromuscular junctions by fluorophore-conjugated α-bungarotoxin labeling (B), and numerical density of phosphorylated neurofilaments (C) compared to ntg littermates. Two-way ANOVA with Tukey's and Sidak's multiple comparison post hoc test; n = 4 per group. Mean + SEM. */^#p < 0.05. (D) Representative images of α-bungarotoxin (white), phosphorylated neurofilaments (pNeurofilaments, red), and protein gene product (PGP) 9.5 (green) triple labeling in the musculus gastrocnemius of a 30 weeks old male SOD1-G93A/low mouse compared to ntg littermate.