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. 2021 Jul 6;35:100837. doi: 10.1016/j.ijcha.2021.100837

Fig. 2.

Fig. 2

Effect of dhSph on the canonical TGFβ pathway. (A) Pre-treatment of NCFs with 1 µM dhSph significantly reduced SMAD2 phosphorylation stimulated by 10 ng/ml TGFβ at 18 min (p < 0.05). (B) Sph (0.1 and 1 µM) had no effect on TGFβ induced phosphorylation of SMAD2. DhSph significantly reduced (C) the 65 kDa latent TGFβ, but (D) had no effect on 45 kDa latent TGFβ, and the (E) 25 kDa mature TGFβ in NCFs treated with the human recombinant TGFβ. (F) and (G) show representative blots. Refer to supplementary figure 2 for original blots. #p < 0.05 vs. TGFβ, **p < 0.01, *p < 0.05 vs. control. Data are presented as ± SEM of 3 replicates.