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. 2021 Jun 24;12:650977. doi: 10.3389/fimmu.2021.650977

Figure 1.

Figure 1

Loss of CDK6 or its kinase activity impairs in vitro expansion of CD8+ T cells. (A, B) Percentage (A) and total numbers (B) of CD3+CD8+ T cells were analyzed by flow cytometry in the spleen of WT, Cdk6- /- and Cdk6 K43M mice (n=8-10). (C) Splenic CD8+ T cells were isolated, activated with αCD3/αCD28 for 48h and cultured in the presence of IL-2. Numbers of CD8+ T cells were analyzed every two days during culture (n=4). (D, E) Cultured CD8+ T cells were kept unstimulated (D) or re-stimulated with αCD3/αCD28 (E) for 24h (n=4). Apoptosis was analyzed by flow cytometric AnnexinV/7-AAD staining and representative dot blots are shown. (A, B, D, E) Bar graphs represent mean ± SEM, pooled from 2-3 independent experiments. (C) Symbols in growth curves represent mean ± SEM, pooled from two independent experiments; ***p < 0.001 (for comparison on d6), two-way ANOVA. ns, not significant.