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. 2021 Jun 24;12:650977. doi: 10.3389/fimmu.2021.650977

Figure 7.

Figure 7

CDK6 regulates negative feedback loops of type I IFN signaling. (A, B) Splenic CD8+ T cells were isolated from Cdk6 fl/fl and Cdk6 fl/fl CD4-Cre mice and activated with αCD3/αCD28 for 48h in the presence of IL-2. Cultured CD8+ T cells were kept unstimulated or stimulated with 10U/ml IFN-β for 2h. Cells were lysed and relative expression levels (normalized to Ube2d2 as a house-keeping gene) of Socs1 (A) and Socs3 (B) were analyzed by qPCR. Relative expression values are shown normalized to unstimulated Cdk6 fl/fl controls (n=6). (C–F) CDK6 ChIP-seq was performed in p185BCR-ABL-transformed B cell lines (12). CDK6 ChIP-seq peaks at the Socs1 (C), Socs3 (D), Mx2 (E) and Tap1 (F) loci are shown. Input samples are shown as controls. Consensus peaks are indicated with lines. (A, B) Bar graphs represent mean ± SEM, pooled from three independent experiments, **p < 0.01, *p < 0.05, paired t-test. ns, not significant.