Figure 2.
miR-155-5p targets and negatively regulates FGF9 expression. (A) miR-155-5p levels in the lung squamous cell carcinoma (SCC) cell lines H2170, H1703, and H520 and the normal bronchial epithelial cell line BEAS-2B were measured by fluorescence quantitative PCR. (B) FGF9 levels in the lung squamous cell carcinoma (SCC) cell lines H2170, H1703, and H520 and the normal bronchial epithelial cell line BEAS-2B were measured by fluorescence quantitative PCR. (C) The binding site of miR-155-5p and FGF9 was predicted. (D) The miR-155-5p and FGF9 3’-UTR region binding site was identified through luciferase reporter gene detection. (E) Western blot was performed to examine how inhibiting miR-155-5p affects FGF9 expression. *P<0.05.
