Figure 1.

Effects of AITC (100 μM), capsaicin (CAPS, 1 μM), OA-NO₂ (30 μM), and OAG (50 μM) on guinea pig spontaneous bladder contractions (SBCs). (A–C) Upper traces show the common excitatory response of AITC and inhibitory responses of CAPS and OA-NO₂. Drugs were applied at the arrows and maintained in the tissue bath. Graphs summarize the major responses to the three agonists as % change in SBC area under the curve (AUC) compared to basal activity, baseline tension (BLT) and the percentage of strips exhibiting the responses. (D–F) Upper traces show the slow onset suppression of SBCs by OAG and the less common excitatory effects of CAPS and OA-NO₂. Graphs summarize the responses of the three agonists as % change in SBC area under the curve (AUC) compared to basal activity, baseline tension (BLT) and the percentage of strips exhibiting the responses. Calibration bars refer to time (min) and tension (gram, g). Statistics were performed on raw numbers: 2-tailed t-test, *P < 0.05 and **P < 0.00, compared with basal activity; n, number of strips.