Figure 2.

Effects of various agents on SBCs in guinea pig bladder strips. (A) Tetrodotoxin (TTX, 1 μM) does not alter SBCs or the inhibitory effect of OA-NO₂ (30μM). The gap (…) in recording represents 24 min. (B) Pretreatment with CAPS (1 μM) blocks the inhibition elicited by the subsequent administration of OA-NO₂ (30 μM) in the same bladder strip. (C) Inhibitory responses of OA-NO₂ (30 μM) and CAPS (1 μM), and the excitatory response of AITC (100 μM) elicited in the same bladder strip by sequential application of the three agents, applied at the arrows and maintained in the tissue bath. (D) A combination of antagonists for TRPV1 (diarylpiperazine, DP, 1 μM) and TRPA1 (HC3-030031, HC3, 10 μM) does not alter the inhibitory effect of OA-NO₂ (30 μM) but suppresses the effects on SBCs of the subsequent administration of CAPS (1 μM) and AITC (100 μM) in the same bladder strip. Calibration bars refer to time (min) and tension (gram, g).