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. 2021 Jun 24;12:686333. doi: 10.3389/fimmu.2021.686333

Figure 1.

Figure 1

Gating Strategy and T-cell composition in AT patients and controls. (A) Overview of the included markers in the antibody panel for spectral flowcytometry grouped per category. (B) Gating strategy for T cells. From left to right: live cells; singlets based on forward scatter; singlets based on side scatter; CD3+ and low side scatter T cells; TCRγδ+ T cells; CD4+ and CD8+ T cells; and finally naive, central memory (CM), effector memory (EM) and terminally differentiated effector memory cell (EMRA) gates within the CD4 and CD8 T cells based on CCR7 and CD45RA expression. (C) Absolute counts of naive, CM, EM and EMRA T cells within the CD4 (left) and CD8 (right) T cells, visualized in bar graphs (median) and individual closed circles of the classic AT (red) and controls (green). Bottom: Pie charts of the percentages of naive, CM, EM and EMRA T cells within the classic AT patients and controls. * means P < 0.05, and ** means P ≤ 0.01.