Table 1.

Levels of sulfite, sulfate, total glutathione, total thiol, total sulfur, and organic detected in Arabidopsis WT, SO Ri, and SiR KD plants are grown on MS media containing 3% sucrose.

Genotype a	Sulfiteb	Sulfatec	Total glutathioned	Total Sulfure	Total thiolf	Organic Sg
	μmol g−1 fresh weight
WT	0.32 ± 0.04b	6.72 ± 0.32b	0.46 ± 0.003a	24.9 ± 0.1b	2.42 ± 0.04b	17.8 ± 0.03b
SO Ri	0.4 ± 0.02a	7.98 ± 0.54ab	0.42 ± 0.002b	24.08 ± 0.6b	2.73 ± 0.02a	15.7 ± 0.02c
SiR KD	0.31 ± 0.01b	8.36 ± 0.77a	0.42 ± 0.003b	28.33 ± 0.2a	2.01 ± 0.04c	20.2 ± 0.01a

Different lowercase letters show significant differences between the genotypes within treatment, as calculated using Tukey–Kramer HSD test JMP8.0 software (http://www.jmp.com/).

^aThe detected metabolite concentrations data are means of n = 3 SE for WT, SO Ri, and SiR KD. The values were normalized by the dry weight content of WT grown under the same 3% sucrose condition and expressed in fresh weight.

^bSulfite was detected by a specific sulfite detection assay using chicken SO (Brychkova et al., 2012a).

^cSulfate was detected by Ion chromatography, as described in material and methods.

^dTotal glutathione was determined according to Griffith (1980) as described in material and methods.

^eTotal sulfur was measured by inductively coupled plasma emission spectrometry (ICP-AES), as described in material methods.

^fTotal thiol was detected using DTNB as described by De Kok et al. (1997).

^gThe organic S was calculated as the difference between total S to the inorganic S (sulfate + sulfite).