Figure 5.

Metabolome-wide association analysis suggests that PCB3 metabolite classes formed in HepG2 cells are significantly associated with several metabolic pathways. The size of circles is proportional to the overlap size (number of significant features) of the pathway enrichment. Circles with black borders are major pathways with >5 significantly associated features. Metabolome-wide association analyses were performed on 18 samples incubated with and without PCB3. Peak areas of the PCB3 metabolites were integrated across all samples and normalized for the total intensities (extracted in the metabolomics analysis) to account for the total mass and recovery effects. Intensities were summed when more than one isomer of each PCB3 metabolite class was detected. For more details about the data analyses, see the Experimental Section. For the number of significantly associated features with each metabolite class, see Figure S13.