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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Bone Miner Res. 2020 Jul 20;35(10):1981–1991. doi: 10.1002/jbmr.4052

Figure 1: Single cell RNA-seq study design.

Figure 1:

(a) Two sets of biologic replicates, each composed of cells that had been isolated CD-1 neonatal mouse calvarium by collagenase-digestion and EDTA treatment (6 calvaria/sample), were collected. An aliquot from each sample was immediately subjected to single cell RNA-seq; the remaining cells were plated, allowed to reach confluence, and cultured under osteogenic conditions for 12 days prior to undergoing single cell RNA-seq. (b) 12-week-old C57Bl/6J male mice received 2 SclAbIII (n=4) or 2 PBS (n=4) injections, 3 days apart. Four days later, tibial and femoral diaphyses were collected from each mouse and the endocortical cells were recovered by collagenase digestion.