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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Bone Miner Res. 2020 Jul 20;35(10):1981–1991. doi: 10.1002/jbmr.4052

Figure 3: Fluorescence activated cell sorting (FACS) and immunocytochemistry analyses detect macrophages among cultured calvarial cells.

Figure 3:

(a) Representative FACS plots indicate a significant expansion of CD45+, F4/80+ macrophages in calvarial cell culture. (b) Fluorescent imaging of cultured calvarial cells at 10x (left) and 20x (right) magnification depict a substantial number of macrophages stained with the F4/80-recognizing antibody (Scale bar: 50um).