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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Bone Miner Res. 2020 Jul 20;35(10):1981–1991. doi: 10.1002/jbmr.4052

Figure 4: Endosteal cell clusters detected by scRNA-seq.

Figure 4:

(a) tSNE plot wherein each dot represents a single cell, and cells sharing the same color code indicate discrete populations of transcriptionally similar cells. Five clusters of cells (highlighted inside the dashed line) represent mesenchymal progenitors and osteoblasts at different stages of differentiation, as indicated by their individual expression profiles depicted in (b) and (c). (b) Top 10 transcript markers corresponding to each of the mesenchymal and endothelial cell clusters as determined by Seurat. (c) Heatmaps of gene expression (left) and associated violin plots (right) indicate cluster-specific expression of representative genes. Increasing expression of Col1a1 and Bglap across clusters #7 and #14 suggest that these clusters represent osteoblasts. Cells in cluster #6 are marked by Pdgfra and Ly6a/Sca1 expression, cluster #21 is marked by Lepr and high levels of Cxcl12 expression, cluster #12 is marked by Ly6a/Sca1 expression, and cluster #19 is marked by Acta2/aSMA expression (Ob: Osteoblast).