Fig. 3.

MEG3 inhibited the expression of RASA1 and activated the RAS-MAPK pathway. a and b Analysis of MEG3-associated proteins using the GSE14722 data and GSE9984 data. c The expression levels of RASA1 in embryonic villi from URSA patients using qRT-PCR. d Correlation between the transcript levels of MEG3 and RASA1 in embryonic villi from URSA patients using qRT-PCR. e Effects of MEG3 on the transcript levels of RASA1 in PT-U cells using qRT-PCR. f Western blot assay: Effects of MEG3 on the RASA1 protein levels in PT-U cells, as well as the levels of Raf, MEK, and ERK proteins (ratio of phosphorylated protein to total protein) in RAS-MAPK pathway. **P < 0.01, *P < 0.05