Fig. 8.

Knockdown of Fmrp and Dcp1a show opposing effects on the cell cycle. Proliferating myoblasts (MB) were treated with siRNAs (Scr, siDcp1a, siFmr1) for 18 h and either induced to enter G0 for 48 h or induced to differentiate for 48 h. For reactivation, G0 cells were harvested and plated on dishes or coverslips for 3 h. a–d EdU incorporation in MB (a), MT (b), G0 (c), and R3 (d): Dcp1a knockdown cells show increased incorporation of EdU in MB, MT, and R3, but not in G0, while Fmrp knockdown cells show decreased incorporation. EdU assay was performed simultaneously for all the conditions. Graphs show quantification by scoring > 500 nuclei for each condition in 3 biological replicates. * p < 0.05, ** p < 0.01. Two-tailed paired Student’s t test was performed. e Consistent with EdU incorporation, Cyclin A2 and Cyclin E show altered protein expression in Dcp1a and Fmrp knockdowns. Gapdh used as internal control. Values represent the mean + SD in 3 biological replicates