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. 2021 Jul 8;18:155. doi: 10.1186/s12974-021-02204-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effect of luteolin treatment on survival and maturation of postmitotic granule neurons in Cdkl5 KO mice. A Examples of sections processed for DCX immunostaining from the dentate gyrus (DG) of an untreated wild-type (+/+), a heterozygous Cdkl5 KO female (+/-), and a 7-day luteolin-treated heterozygous Cdkl5 KO female (10 mg/Kg; +/- Lut 7d) mouse. B Number of DCX-positive cells in the DG of untreated Cdkl5 +/+ (n=6) and Cdkl5 +/- (n=6) mice, and 7-day luteolin-treated Cdkl5 +/- mice (Lut +, n=4). Data are expressed as number of DCX positive cells per 100 μm of granule cell layer. C Number of Ki-67-positive cells in the DG of mice as in B. D Mean total dendritic length of CA1 pyramidal neurons of mice as in B. Values in B, C, and D are represented as means ± SEM. *p< 0.05, **p< 0.01 (Fisher’s LSD test after one-way ANOVA). E, F Western blot analysis of BDNF and GAPDH levels in somatosensory cortex homogenates from untreated Cdkl5 mice (+/+ n=4, +/- n=4) and 7-day luteolin-treated Cdkl5 +/- mice (+/- Lut7d, n=4). Examples of immunoblot in E. Histogram in F shows mature BDNF protein levels normalized to GAPDH. Data are expressed as a percentage of untreated Cdkl5 +/+ mice. Values are represented as means ± SEM of two technical replicates; *p<0.05;**p < 0.01 (Fisher’s LSD test after one-way ANOVA)