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. 2021 Jul 7;40:226. doi: 10.1186/s13046-021-01982-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — RANBP3L deficiency induces cell migration and colony forming capacity. A Representative images of the migration assay. Scr, R1 and R3 mpkCCD cells were cultivated in 96 well plates and cell migration was monitored by live cell imaging using IncuCyte S3 system. The pictures show the progressive closure of an induced wound at indicated time points (0 h and 8 h). Wound areas are labeled in dark green. Scale bar: 400 μm. B Plot of the wound density over time. Migration assay was performed for 24 h. Values represent mean ± SEM (error bars). n = 6 (with 4 technical replicates). C The relative migration change after 8 h was calculated by linear regression analysis using GraphPad Prism. ***, p < 0.001, 1-way ANOVA. D Representative images of Scr, R1 and R3 cells during colony forming assay. After 2 weeks of incubation 4–5 images per well were taken and stacked with the image J software tool. Scale bar: 500 pixels. E-F Colony number and colony size at 300 mosmol/kg (E) and 600 mosmol/kg (F). Values are represented in a whiskers 1–99 percentile plot. n.d. = not detectable, *, p < 0.05, **, p < 0.01, ***, p < 0.001, 1-way ANOVA