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. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: Neuron. 2020 Aug 7;108(1):164–179.e7. doi: 10.1016/j.neuron.2020.07.012

Figure 4: Comparison of the effects of loss of Bmal1 on Coupling in VIP and AVP neurons in the SCN.

Figure 4:

(A, B) Pairwise MIC score plots of neurons in the VIP SCN versus the VIP-Bmal1−/− (cKO) SCN, during pretreatment, TTX treatment, and washout. As annotated in D, horizontal and vertical dashed lines split the plot into four parts, including the lower-left part showing the pair-wise MIC value among green cells, lower-right part is MIC values of green-red pairs and higher-right part is red-red pairs. Correlation network was constructed from pairwise MIC analysis: A connection line was drawn when the MIC value of the cell pair is larger than 0.8. Right-left SCN associations are not shown. (C) Mean +/− SEM MIC scores are shown to compare three types of associations between VIP-Cre and VIP-Bmal1−/− SCNs. The associations were categorized as green-green (or KO-KO), red-red (or Cre-Cre) and green-red (or KO-Cre). Each mean MIC value from a replicate is represented by a point in the overlaid scatter plot (VIP, n=3; VIP-Bmal1−/−, n=4). Higher asterisks represent the significance of genotype factor in two-way ANOVA, and lower asterisks represent significance of Holm-Sidak’s multiple comparisons. (E, F) Pairwise MIC score plots of neurons in the AVP SCN versus the AVP-Bmal1−/− SCN, during pretreatment, TTX treatment, and washout. (G ) Mean +/− SEM of MIC scores are shown to compare three types of associations (as in C) between AVP-Cre and AVP-Bmal1−/− SCNs (AVP, n=5; AVP-Bmal1−/−, n=4). Asterisks indiciate significant differences (* p ≤0.05; ** p ≤ 0.01; *** p ≤ 0.001). Detailed quantification results are included in Supplemental Data 1.