Skip to main content
. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: Neuron. 2020 Aug 7;108(1):164–179.e7. doi: 10.1016/j.neuron.2020.07.012

Figure 5: Immunofluorescent staining of key components of VIP and AVP signaling in VIP-Bmal1−/−, AVP-Bmal1−/− and VIP/AVP-Bmal1−/− SCNs.

Figure 5:

(A - D) Immunofluorescent images from 50μm thick SCN slices from wildtype control, VIP-Bmal1−/− (cKO), AVP-Bmal1−/−(cKO) and VIP/AVP-Bmal1−/− (cKO) SCNs, stained with AVP, VIP, AVP receptor V1a and VIP receptor VPAC2 antibodies. Green signals from the neuropeptides or receptors are merged with the blue staining of nuclear DNA. Scale bars represent 100μm. (E-H) Mean +/− SEM plots of fluorescence intensity normalized to wildtype, VIP-Cre and AVP-Cre controls (one of each) are shown as the relative abundance. Each replicate is shown as a point in the overlaid scatter plots (all n=3). Asterisks indicate the significance of Holm-Sidak’s comparisons to controls and ANOVA adjusted p-value (* p ≤0.05; ** p ≤ 0.01; *** p ≤ 0.001).