Table 1.

Selected features

Selected features for PMO			Selected features for 2OMe
Name	Description	FIa	Name	Description	FIa
ASO concentrationb	Concentration of oligomer used in the experiment	0.64±0.14	ASO concentrationb	Concentration of oligomer used in the experiment	0.11±0.05
Exon v intron %GC after blocking by oligo	%GC in exon when blocked by oligo / %GC 5′ intron 200 bases upstream	0.68±0.15	GCs (number of)	Total GCs in ASO sequence	0.67±0.20
dG (50BaseFlanksAroundTarget)	Predicted binding energy (21) of ASO to the target sequence plus 50-base flanks (13)	0.66±0.16	ACP	Distance in bases from the splice acceptor site to the center of the target site (17)	0.49±0.21
ACC_LAST15	Predicted accessibility scores (22) of the 3′ end of the target (last 15 bases)	0.32±0.09	%GC of exon when blocked by oligo	Total remaining %GCs of target exon sequence when blocked by ASOs	0.46±0.11
			niscore_per_base	Cumulative NI score (24) divided by the number of exon bases.	0.18±0.09
			ACC_LAST8	Predicted accessibility scores of the 3′ end of the target (last 8 bases)	0.12±0.07

^aThe feature importance (FI) was calculated by the permutation importance method (23).

^bThe ASO concentration used in the experiment is always included as one of the features of the predictive model.