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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Nat Med. 2021 Mar 18;27(4):677–687. doi: 10.1038/s41591-021-01284-y

Extended Data Fig. 4 ∣. Staining and gating scheme used to analyze editing and differentiation rates of RBCs.

Extended Data Fig. 4 ∣

a, Representative flow cytometry staining and gating scheme for human HSPCs targeted at HBA1 with HBB-T2A-YFP (HBA1 UTRs) and differentiated into RBCs. This indicates that only RBCs (CD34−/CD45−/CD71+/GPA+) are able to express the promoterless YFP marker. Analysis was performed on BD FACS Aria II platform. b, Representative flow cytometry images of RBCs (CD34−/CD45−/CD71+/GPA+) derived from HSPCs targeted with HBA1 UTRs, HBA2 UTRs, and HBB UTRs vector. AAV only controls were used for each vector to establish gating scheme, leading to slight variation in positive/negative cut-offs across images.