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. 2021 Apr 10;2(4):370–387. doi: 10.1158/2643-3230.BCD-20-0108

Figure 1.

Figure 1.

KDM5 mediates multiple myeloma (MM) cell growth. A and B, Violin plots depicting KDM5A (blue), KDM5B (red), and KDM5C (green) expression in primary multiple myeloma samples (GSE2658, n = 559; A) and multiple myeloma cell lines (CCLE, n = 20; B). Center dashed lines represent the median, and lower and upper dashed lines represent the interquartile range. TPM, transcripts per million. C, Immunoblot analysis for KDM5A, KDM5B, and KDM5C in multiple myeloma cell lines, normal B cells, and multiple myeloma patient samples. MCF7 cells served as positive control for KDM5A and KDM5B expression levels. GAPDH served as loading control. D, Multiple myeloma cell lines were transduced with shKDM5A, shKDM5B, shKDM5C, or control shRNA targeting luciferase (shLuc). Three days after infection is designated as day 0. On day 0, whole-cell lysates were extracted and subjected to immunoblot analysis for KDM5A, KDM5B, and KDM5C. GAPDH served as loading control (top). Viable cells were measured by MTT assay on days 0 and 5. The cell growth rate (day 5/day 0) relative to shLuc is shown (bottom). Data represent mean ± SD of quadruplicate cultures. ***, P < 0.001 compared with shLuc; unpaired Student t test.