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. Author manuscript; available in PMC: 2021 Jul 8.
Published in final edited form as: Cell Rep. 2021 Jun 15;35(11):109267. doi: 10.1016/j.celrep.2021.109267

Figure 5. Spn binds to dying cell on mouse lung.

Figure 5.

Mice were treated intratracheally with rPly (160 ng/mouse) or PBS. After 24 h, mice were challenged intratracheally with (A) FITC-rPspA or(B) 106 CFUs of FITC-labeled Spn EF3030 (WT, pspA mutant [ΔpspA], or pspA revertant [ΔpspA;pspA]) and then euthanized after 1 h (n = 5 per cohort). Lungs were collected and co-stained with anti-pMLKL (red) and DAPI (blue). White arrows indicate Spn. Mean fluorescence intensity (MFI) of the FITC-labeled rPspA (green) or Spn (green) was measured using ImageJ. Kruskal-Wallis test with Dunn’s multiple-comparison post-test. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001. Scale bars: 50 μm for all microscopic images.